Differential Effects of Overexpressed Glucokinase and Hexokinase I in Isolated Islets: EVIDENCE FOR FUNCTIONAL SEGREGATION OF THE HIGH AND LOW Km …
TC Becker, RJ Noel, JH Johnson, RM Lynch… - Journal of Biological …, 1996 - ASBMB
Glucose-stimulated insulin secretion is believed to require metabolism of the sugar via a
high K m pathway in which glucokinase (hexokinase IV) is rate-limiting. In this study, we
have used recombinant adenoviruses to overexpress the liver and islet isoforms of
glucokinase as well as low K m hexokinase I in isolated rat islets of Langerhans. Glucose
phosphorylating activity increased by up to 20-fold in extracts from islets treated with
adenoviruses containing the cDNAs encoding either tissue isoform of glucokinase, but such …
high K m pathway in which glucokinase (hexokinase IV) is rate-limiting. In this study, we
have used recombinant adenoviruses to overexpress the liver and islet isoforms of
glucokinase as well as low K m hexokinase I in isolated rat islets of Langerhans. Glucose
phosphorylating activity increased by up to 20-fold in extracts from islets treated with
adenoviruses containing the cDNAs encoding either tissue isoform of glucokinase, but such …
