Isolation and characterization of the distal promoter region of mouse Cbfa1
M Fujiwara, S Tagashira, H Harada, S Ogawa… - … et Biophysica Acta (BBA …, 1999 - Elsevier
Cbfa1 is an essential transcription factor for bone formation, and as such little is known
about the region responsible for the transcriptional regulation of this gene. Here we report
the determination of the transcription start sites, isolation and partial characterization of distal
promoter region of this gene. Three transcription start sites were identified by the 5′-Cap
site method, recently invented for rapid examination of the 5′-end of genes of interest. A
reporter construct containing 1.8 kb of 5′ of transcription start sites had∼ 25-fold more …
about the region responsible for the transcriptional regulation of this gene. Here we report
the determination of the transcription start sites, isolation and partial characterization of distal
promoter region of this gene. Three transcription start sites were identified by the 5′-Cap
site method, recently invented for rapid examination of the 5′-end of genes of interest. A
reporter construct containing 1.8 kb of 5′ of transcription start sites had∼ 25-fold more …
