Stable triple helices are formed upon binding of RNA oligonucleotides and their 2'-O-methyl derivatives to double-helical DNA.
C Escude, JS Sun, M Rougee, T Garestier… - Comptes Rendus de L' …, 1992 - europepmc.org
C Escude, JS Sun, M Rougee, T Garestier, C Hélène
Comptes Rendus de L'academie des sciences. Serie III, Sciences de la vie, 1992•europepmc.orgPyrimidine oligoribonucleotides bind to the major groove of double-helical DNA at
homopurine. homopyrimidine sequences. They recognize Watson-Crick base pairs by
forming TA x U and CG x C base triplets via Hoogsteen hydrogen bonding. The stability of
these triple helices is much higher than that of triple helices formed by
oligodeoxyribonucleotides as shown by an increase of the temperature at which half-
dissociation of the third strand occurs. When the 2'-hydroxyl group of ribose moieties is …
homopurine. homopyrimidine sequences. They recognize Watson-Crick base pairs by
forming TA x U and CG x C base triplets via Hoogsteen hydrogen bonding. The stability of
these triple helices is much higher than that of triple helices formed by
oligodeoxyribonucleotides as shown by an increase of the temperature at which half-
dissociation of the third strand occurs. When the 2'-hydroxyl group of ribose moieties is …
Pyrimidine oligoribonucleotides bind to the major groove of double-helical DNA at homopurine. homopyrimidine sequences. They recognize Watson-Crick base pairs by forming TA x U and CG x C base triplets via Hoogsteen hydrogen bonding. The stability of these triple helices is much higher than that of triple helices formed by oligodeoxyribonucleotides as shown by an increase of the temperature at which half-dissociation of the third strand occurs. When the 2'-hydroxyl group of ribose moieties is replaced by 2'-O-methyl substituent, triple helix stability is further increased.
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