Extreme instability of pyrimidine motif triplex DNA at physiological pH severely limits its use
in an artificial control of gene expression in vivo. Stabilization of the pyrimidine motif triplex
at physiological pH is, therefore, crucial in improving its therapeutic potential. To this end, we
have investigated the thermodynamic and kinetic effects of our previously reported chemical
modification, 2′-O, 4′-C-methylene bridged nucleic acid (2′, 4′-BNA) modification of
triplex-forming oligonucleotide (TFO), on pyrimidine motif triplex formation at physiological …