[HTML][HTML] Colony-stimulating factor-1 impairs both proliferation and differentiation signals of erythropoietin during the commitment of bipotential NFS-60 cell line to the …
G Pawlak, C Valadoux-Delplanque, V Revol… - Experimental …, 1999 - Elsevier
The interleukin-3 (IL-3) dependent cell line NFS-60 contains bipotential progenitors that
exhibit both erythroid and myelomonocytic potentials. In order to study their commitment to
the monocytic lineage, NFS-60 cells were retrovirally transduced with mouse c-fms cDNA,
which encodes the colony-stimulating factor-1 receptor (CSF-1R), resulting in the N-Fms cell
line. N-Fms cells proliferated in response to CSF-1 with a growth rate similar to that obtained
in response to IL-3 and progressively differentiated from myeloid blasts to monocytic cells …
exhibit both erythroid and myelomonocytic potentials. In order to study their commitment to
the monocytic lineage, NFS-60 cells were retrovirally transduced with mouse c-fms cDNA,
which encodes the colony-stimulating factor-1 receptor (CSF-1R), resulting in the N-Fms cell
line. N-Fms cells proliferated in response to CSF-1 with a growth rate similar to that obtained
in response to IL-3 and progressively differentiated from myeloid blasts to monocytic cells …
