Long-term culture of infant leukemia cells: dependence upon stromal cells from the bone marrow and bilineage differentiation
T Umiel, S Friedman, R Zaizov, IJ Cohen, Y Gozes… - Leukemia research, 1986 - Elsevier
Infant leukemia cells with 46XY, t (11; 17)(q23; p13) karyotype and a hybrid pre B myeloid
phenotype (HLA-DR,(Ia), B4 and My7-positive and CALLA and T11-negative) and
immunoglobulin heavy chain gene rearrangement were maintained in long-term culture for
over 10 months. The in-vitro survival and growth of the leukemia cells were strictly
dependent upon the presence of their autologous marrow stromal cells. The latter could be
replaced by the 14F1. 1 clone of preadipocytes derived from mouse bone marrow. Neither …
phenotype (HLA-DR,(Ia), B4 and My7-positive and CALLA and T11-negative) and
immunoglobulin heavy chain gene rearrangement were maintained in long-term culture for
over 10 months. The in-vitro survival and growth of the leukemia cells were strictly
dependent upon the presence of their autologous marrow stromal cells. The latter could be
replaced by the 14F1. 1 clone of preadipocytes derived from mouse bone marrow. Neither …
