CCL22 recruits CD4-positive CD25-positive regulatory T cells into malignant pleural effusion
XJ Qin, HZ Shi, JM Deng, QL Liang, J Jiang… - Clinical Cancer Research, 2009 - AACR
XJ Qin, HZ Shi, JM Deng, QL Liang, J Jiang, ZJ Ye
Clinical Cancer Research, 2009•AACRPurpose: The aim of this study was to explore the presence of the chemokines CCL22 and
CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines
on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural
space. Experimental Design: The concentrations of CCL22 and CCL17 in both pleural
effusions and sera from 33 patients with lung cancer were determined. Flow cytometry was
done to determine T lymphocyte subsets in cell pellets of pleural effusion. Pleural cells were …
CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines
on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural
space. Experimental Design: The concentrations of CCL22 and CCL17 in both pleural
effusions and sera from 33 patients with lung cancer were determined. Flow cytometry was
done to determine T lymphocyte subsets in cell pellets of pleural effusion. Pleural cells were …
Abstract
Purpose: The aim of this study was to explore the presence of the chemokines CCL22 and CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural space.
Experimental Design: The concentrations of CCL22 and CCL17 in both pleural effusions and sera from 33 patients with lung cancer were determined. Flow cytometry was done to determine T lymphocyte subsets in cell pellets of pleural effusion. Pleural cells were analyzed for the expression of CCL22 and CCL17. The chemoattractant activity of CCL22 for regulatory T cells in vitro and in vivo was also observed.
Results: The concentration of CCL22 in malignant pleural effusion was significantly higher than that in the corresponding serum. Pleural fluid from lung cancer patients was chemotactic for regulatory T cells, and this activity was partly blocked by an anti-CCL22, but not by an anti-CCL17 antibody. Intrapleural administration of CCL22 of patients produced a marked progressive influx of regulatory T cells into pleural space.
Conclusions: Compared with serum, CCL22 seemed to be increased in malignant pleural effusion, and could directly induce regulatory T cell infiltration into the pleural space in patients with malignant effusion.
AACR
