Determination of tumor necrosis factor-alpha levels in dengue virus infected patients by sensitive biotin-streptavidin enzyme-linked immunosorbent assay
L Kittigul, W Temprom, D Sujirarat, C Kittigul - Journal of virological …, 2000 - Elsevier
A modified sandwich enzyme-linked immunosorbent assay using biotin-streptavidin system
(BS-ELISA) was developed to determine levels of tumor necrosis factor-alpha (TNF-α) in
serum samples of children infected with dengue virus (n= 99) and healthy controls (n= 41).
The minimum detectable concentration of TNF-α by the BS-ELISA was 3.3 pg/ml. The mean
TNF-α level was highest in those patients with dengue shock syndrome (DSS) or dengue
hemorrhagic fever (DHF) grade III (37.44±42.0 pg/ml). Lower levels were found in DHF …
(BS-ELISA) was developed to determine levels of tumor necrosis factor-alpha (TNF-α) in
serum samples of children infected with dengue virus (n= 99) and healthy controls (n= 41).
The minimum detectable concentration of TNF-α by the BS-ELISA was 3.3 pg/ml. The mean
TNF-α level was highest in those patients with dengue shock syndrome (DSS) or dengue
hemorrhagic fever (DHF) grade III (37.44±42.0 pg/ml). Lower levels were found in DHF …
