[HTML][HTML] A NOVel ELISPOT assay to quantify HLA-specific B cells in HLA-immunized individuals
S Heidt, DL Roelen, YJH de Vaal, MGD Kester… - American journal of …, 2012 - Elsevier
Quantification of the humoral alloimmune response is generally achieved by measuring
serum HLA antibodies, which provides no information about the cells involved in the
humoral immune response. Therefore, we have developed an HLA-specific B-cell ELISPOT
assay allowing for quantification of B cells producing HLA antibodies. We used recombinant
HLA monomers as target in the ELISPOT assay. Validation was performed with human B-
cell hybridomas producing HLA antibodies. Subsequently, we quantified B cells producing …
serum HLA antibodies, which provides no information about the cells involved in the
humoral immune response. Therefore, we have developed an HLA-specific B-cell ELISPOT
assay allowing for quantification of B cells producing HLA antibodies. We used recombinant
HLA monomers as target in the ELISPOT assay. Validation was performed with human B-
cell hybridomas producing HLA antibodies. Subsequently, we quantified B cells producing …