Cholinergic interneurons are the only known source of acetylcholine in the rat nucleus
accumbens (nAcb); yet there is little anatomical data about their mode of innervation and the
origin of their excitatory drive. We characterized the cholinergic and thalamic innervations of
nAcb with choline acetyltransferase (ChAT) immunocytochemistry and anterograde transport
of Phaseolus vulgaris-leucoagglutinin (PHA-L) from the midline/intralaminar/paraventricular
thalamic nuclei. The use of a monoclonal ChAT antiserum against whole rat ChAT protein …