Cholera toxin induces cAMP-independent degradation of Gs
FH Chang, HR Bourne - Journal of Biological Chemistry, 1989 - ASBMB
Cholera toxin stimulates adenylyl cyclase by catalyzing ADP-ribosylation of the αchain (α s)
of G s, a guanine nucleotide binding regulatory protein. In a rat pituitary cell line, GH3, the
toxin-induced increase in GTP-dependent adenylyl cyclase activity is maximal at 1 h;
adenylyl cyclase remains elevated for at least 32 h. Surprisingly, cholera toxin also induces
a 74–95% decrease in the amount of immunoreactive α s in the same cells, as assessed on
immunoblots probed with either of two antisera directed against separate α s peptide …
of G s, a guanine nucleotide binding regulatory protein. In a rat pituitary cell line, GH3, the
toxin-induced increase in GTP-dependent adenylyl cyclase activity is maximal at 1 h;
adenylyl cyclase remains elevated for at least 32 h. Surprisingly, cholera toxin also induces
a 74–95% decrease in the amount of immunoreactive α s in the same cells, as assessed on
immunoblots probed with either of two antisera directed against separate α s peptide …