RNA-guided, engineered nucleases derived from the prokaryotic adaptive immune system
CRISPR-Cas represent a powerful platform for gene deletion and editing. When used as a
therapeutic approach, direct delivery of Cas9 protein and single-guide RNA (sgRNA) could
circumvent the safety issues associated with plasmid delivery and therefore represents an
attractive tool for precision genome engineering. Gene deletion or editing in adipose tissue
to enhance its energy expenditure, fatty acid oxidation, and secretion of bioactive factors …