[HTML][HTML] Control of insulin mRNA stability in rat pancreatic islets: regulatory role of a 3′-untranslated region pyrimidine-rich sequence
L Tillmar, C Carlsson, N Welsh - Journal of Biological Chemistry, 2002 - ASBMB
Stabilization of insulin mRNA in response to glucose is a significant component of insulin
production, but the mechanisms governing this process are unknown. We presently observe
that insulin mRNA is a highly abundant messenger and that the content of this mRNA is
mainly controlled by changes in messenger stability. We also demonstrate specific binding
of the polypyrimidine tract-binding protein to a pyrimidine-rich sequence located in the 3′-
untranslated region (3′-UTR) of insulin mRNA. This binding was increased in vitro by …
production, but the mechanisms governing this process are unknown. We presently observe
that insulin mRNA is a highly abundant messenger and that the content of this mRNA is
mainly controlled by changes in messenger stability. We also demonstrate specific binding
of the polypyrimidine tract-binding protein to a pyrimidine-rich sequence located in the 3′-
untranslated region (3′-UTR) of insulin mRNA. This binding was increased in vitro by …