[CITATION][C] Evidence for the common identity of glucose 6-phosphatase, inorganic pyrophosphatase, and pyrophosphate-glucose phosphotransferase
RC Nordlie, WJ Arion - Journal of Biological Chemistry, 1964 - Elsevier
RC Nordlie, WJ Arion
Journal of Biological Chemistry, 1964•ElsevierRESULTS Fractionation Studies-A 15 to 2-fold increase in specific activity of glucose 6-
phosphatase, inorganic pyrophosphatase, and PP;-glucose phosphotransferase was
achieved routinely by ammonium sulfate fractionation of deoxycholate-treated microsomes
(Fig. 1 depicts a typical experiment). A partial separation of these activities from ATPase,
Mg++-stimulated inorganic pyrophosphatase(pH 7.4), and acid phosphatase also was
achieved. The glucose 6-phosphatase to phosphotransferase ratio remained nearly …
phosphatase, inorganic pyrophosphatase, and PP;-glucose phosphotransferase was
achieved routinely by ammonium sulfate fractionation of deoxycholate-treated microsomes
(Fig. 1 depicts a typical experiment). A partial separation of these activities from ATPase,
Mg++-stimulated inorganic pyrophosphatase(pH 7.4), and acid phosphatase also was
achieved. The glucose 6-phosphatase to phosphotransferase ratio remained nearly …
RESULTS
Fractionation Studies-A 15 to 2-fold increase in specific activity of glucose 6-phosphatase, inorganic pyrophosphatase, and PP;-glucose phosphotransferase was achieved routinely by ammonium sulfate fractionation of deoxycholate-treated microsomes (Fig. 1 depicts a typical experiment). A partial separation of these activities from ATPase, Mg++-stimulated inorganic pyrophosphatase(pH 7.4), and acid phosphatase also was achieved. The glucose 6-phosphatase to phosphotransferase ratio remained nearly constant (3.4 in deoxycholate-treated microsomes; 2.8 in Fraction I; 3.2 in Fraction II; 3.5 in Fraction III; and 3.2 in Fraction IV). Inorganic pyrophosphatase activity at
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