[HTML][HTML] Complete genomic sequence of Epstein-Barr virus in nasopharyngeal carcinoma cell line C666-1
KKY Tso, KYL Yip, CKY Mak, GTY Chung… - Infectious agents and …, 2013 - Springer
Infectious agents and cancer, 2013•Springer
Background Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is
consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro
native EBV-infected NPC cell model commonly used for study of the viral-host interaction.
Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model
has not been characterized. Objective To determine the complete EBV genome sequence in
C666-1 cells. Methods The C666-1 genome was sequenced by 100-bases pair-end …
consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro
native EBV-infected NPC cell model commonly used for study of the viral-host interaction.
Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model
has not been characterized. Objective To determine the complete EBV genome sequence in
C666-1 cells. Methods The C666-1 genome was sequenced by 100-bases pair-end …
Background
Nasopharyngeal carcinoma is a distinct type of head and neck cancer which is consistently associated with Epstein-Barr virus (EBV). The C666-1 cell line is the only in vitro native EBV-infected NPC cell model commonly used for study of the viral-host interaction. Nevertheless, the complete EBV genome sequence in this in vitro EBV-infected NPC model has not been characterized.
Objective
To determine the complete EBV genome sequence in C666-1 cells.
Methods
The C666-1 genome was sequenced by 100-bases pair-end massive parallel sequencing. Bioinformatics analysis was performed to extract the EBV sequences and construct an EBV consensus sequence map. PCR amplification and Sanger DNA sequencing were used for sequence validation and gap filling. A phylogenetic analysis of EBV strain in C666-1 cells and other reported EBV strains was performed.
Results
A 171,317 bp complete EBV genome of C666-1 was successfully constructed (GenBank accession number: KC617875). Phylogenetic analysis of EBV genome in C666-1 revealed that the C666-1 EBV strain is closely related to the reported strains in NPC primary tumors.
Conclusion
C666-1 contains a representative NPC-associated EBV genome and might serve as an important model for studying the roles or function of viral proteins in NPC tumorigenesis.
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